To learn how to make an artificial heart, researchers have created artificial fish from human cardiac muscle cells.

The fish are made from human cardiac muscle cells, painstakingly grown on laboratory tape coated with gelatin.

“Our ultimate goal is to build an artificial heart…”

The “biohybrid” fish swim by recreating the muscle contractions of a pumping heart, bringing engineers closer to creating more complex artificial pumps and providing a platform to study human diseases, such as cardiac arrhythmias.

Biomedical engineer Sung-Jin Park began working on biohybrid fish as a postdoc in Kit Parker’s lab at Harvard’s Paulson School of Engineering and Applied Sciences. Park is co-first author of a paper in Science.

“Our major achievement in this paper is that we can build a living muscle that can self-control and self-pace its movement without any additional control mechanism,” says Park, now an assistant professor in the biomedical engineering department at Georgia Tech and Emory and part of the Multi-Cellular Engineered Living Systems (M-CELS) program.

Park says his team’s research could be applied to design “biological pacemakers,” a potential alternative to electronic cardiac pacemakers. As part of a long-term implant, biological pacemakers could grow along with a pediatric patient.

“Our ultimate goal is to build an artificial heart to replace a malformed heart in a child,” says Parker, senior author of the paper. “Rather than using heart imaging as a blueprint, we are identifying the key biophysical principles that make the heart work, using them as design criteria, and replicating them in a system—a living, swimming fish—where it is much easier to see if we are successful.”

The biohybrid fish even improves with age.

The fish has two layers of muscle cells, one on each side of the tail fin. When one side contracts, the other stretches. The stretch triggers the opening of an ion channel sensitive to mechanical movement, leading to an influx of charged ions and a contraction on that side.

The researchers also engineered an “autonomous pacing node,” acting like a pacemaker, which controls the frequency and rhythm of the spontaneous contractions. Together, the two layers of muscle and the autonomous pacing node enable the generation of continuous, spontaneous, and coordinated, back-and-forth fin movements. The system can propel the fish for more than 100 days.

In addition, the biohybrid fish improves with age. Its muscle contraction strength, maximum swimming speed, and muscle coordination all increase for the first month as the cardiomyocyte cells mature. Eventually, the biohybrid fish reached speeds similar to a model organism (zebrafish) in the wild.

The biohybrid fish builds off previous research from Parker’s lab. In 2012, the Harvard team used cardiac muscle cells from rats to build a jellyfish-like biohybrid pump and in 2016 Park and his colleagues developed a swimming, artificial stingray, also from rat heart muscle cells.

The team’s discovery of the self-generated stretch/contract cycles was partly fortuitous.

“We did not expect that the mechano-electric coupling effect would be strong enough to produce our fish’s antagonistic muscle contractions,” Park says. “In the beginning, we used light to control fish movements, like what we did for our stingray. One day, after finishing this experiment, we saved the fish in the incubator for more experiments but totally forgot the fact that we saved the fish. After a couple of weeks, when we opened the incubator, we found out that the fish swam with its own pacing by itself.”

Park says he is eager to work on models of cardiac arrhythmias (sinoatrial node dysfunctions) and wants to investigate mechano-electrical signaling as a therapeutic target of heart rhythm management.

The human cardiac muscle cells used in this research are commercially available. They come from induced pluripotent stem cells, which are originally derived from skin cells.

Additional researchers from Harvard and Emory/Georgia Tech contributed to the work.

Support for the research came from the National Center for Advancing Translational Sciences and the National Science Foundation.

Source: Emory University